MITOCHONDRIAL INCORPORATION OF TRITIATED THYMIDINE IN TETRAHYMENA PYRIFORMIS
نویسندگان
چکیده
منابع مشابه
Mitochondrial Incorporation of Tritiated Thymidine in Tetrahymena Pyriformis
Cytoplasmic D N A is known in a number of cell types. Hoff-JCrgenson and Zeuthen (7), using a microbial bioassay method, reported the presence of cytoplasmic D N A in frog eggs. Later, Hoff-J~6rgenson (8) and Elson et al. (4) indicated that sea urchin eggs contained a D N A reserve in the cytoplasm. Using autoradiographs of whole cells, Scherbaum (21) described cytoplasmic labeling in Ha-thymid...
متن کاملMitochondrial and Cytoplasmic Ribosomes from Tetrahymena Pyriformis
Mitochondrial and cytoplasmic ribosomes from Tetrahymena pyriformis have been isolated and studied by the techniques of polyacrylamide gel electrophoresis and electron microscopy used in conjunction. Although the two ribosome types show the same coefficient of sedimentation (80S) in sucrose gradients, they can be distinguished by gel electrophoresis: mitoribosomes migrate in a single band, cons...
متن کاملFatty acids of mitochondrial membranes from Tetrahymena pyriformis.
We have examined the fatty acid composition of the mitochondrial membranes in three strains of Tetrahymena pyriformis. All three had similar components and exhibited large amounts of unsaturated fatty acids. The cytoplasmic mutant, CA-10, which has a slower growth rate and unusual membrane morphology, had a slightly higher amount of iso-acids but was otherwise similar to the other strains in fa...
متن کاملLa Synthèse De L'adn Mitochondrial Chez Tetrahymena Pyriformis
Electron microscopic radioautography has been used to study the synthesis of mitochondrial DNA after incorporation of thymidine-(3)H by cultures in logarithmic phase of Tetrahymena pyriformis during periods ranging from 15 min to 12 hr. The great majority of silver grains are distributed over the macronuclei, the micronuclei, and the mitochondria. The intensity of the label over the entire mito...
متن کاملRibosome Synthesis in Tetrahymena Pyriformis
The cellular site of synthesis of ribosomal RNA in Tetrahymena pyriformis was studied by analyzing the purified nuclear and cytoplasmic RNA from cells pulse labeled with uridine-(3)H. The results of studies using zonal centrifugation in sucrose density gradients show that the ribosomal RNA is synthesized in the nucleus as a large precursor molecule sedimenting at 35S. The 35S molecule undergoes...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Cell Biology
سال: 1965
ISSN: 1540-8140,0021-9525
DOI: 10.1083/jcb.25.3.641